P-113: Survey of In Vitro Effect of Resveratrol on Nitric Oxide Secretion of Human Endometrial Epithelial Cells
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Abstract:
Background: Resveratrol is a natural polyphe- nolic compound, synthesized by plants as a phytoalexin and protects against ultraviolet radiation and fungal infection. Nitric oxide is formed from L-arginine through nitric oxide synthases (NOS), a group of enzymes that structurally resemble cytochrome P-450 reductase. In females, circulating NO is increased during follicle development and decreased immediately after ovulation. The aim of present study was to determine in vitro resveratrol effect on nitric oxide (NO) secretion by human endometrial epithelial cells. Materials and Methods: In this experiment study, human endometrial biopsies (n=8) were chopped in sterile conditions. Epithelial cells, were isolated after enzymatic digestion (collagenase 2 mg/ml) and cell filtration though filter mesh. Cell viability was defined by trypan blue staining. The cells were divided into five groups: control, 1,10, 20 and 50 μM resveratrol concentration for three culture periods(24,48,72 hours). Nitric oxide secretion were assessed by Geriss assay. Data was analyzed by one way ANOVA and p<0.05 was considered significant. Results: Difference between NO amount were significant in control, 1, 10, 20 and 50 μM resveratrol concentration were 9.08 ± 1.6, 9.62 ± 1.7, 11 ± 1.6, 12.78 ± 2.1, 20.2 ± 5.1 μM at 24- hour culture period (p=0.08), 11.94 ± 1.3, 13.61 ± 1.06, 15.91 ± 1.2, 16.64 ± 1.5, 25.63 ± 5.4 μM at 48 hours (p=0.04), 13.81 ± 1.83, 16.06 ± 1.14, 16.43 ± 1.08, 17.45 ± 1.6, 26.74 ± 5.6 μM at 72 hours (p=000) respectively. Conclusion: Resveratrol increased endometrial epithelial cells NO secretion in dose dependent manner. Its higher doses showed significant effect.
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Journal title
volume 8 issue 2.5
pages 126- 126
publication date 2014-07-01
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